5YRO

RanL182A in complex with RanBP1-CRM1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-03-15
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97921
Spacegroup name	P 43 21 2
Unit cell lengths	105.259, 105.259, 307.425
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.531 - 2.396
R-factor	0.2091
Rwork	0.207
R-free	0.24460
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4hb4
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.440
High resolution limit [Å]	2.396	6.510	2.400
Rmerge	0.167	0.135
Rmeas	0.179	0.145
Rpim	0.070	0.051
Number of reflections	65435	3447	3233
<I/σ(I)>	5
Completeness [%]	94.8	90.2	95.4
Redundancy	9	7.9	8.9
CC(1/2)		0.979	0.410

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.6	293	18% PEG 3350, 200mM Ammonium Nitrate, 100mM Bis-Tris pH 6.6