5YQB

Crystal structure of E.coli aminopeptidase N in complex with Puromycin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2016-12-10
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54
Spacegroup name	P 31 2 1
Unit cell lengths	120.292, 120.292, 170.123
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 1.560
R-factor	0.16239
Rwork	0.161
R-free	0.18189
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2hpo
RMSD bond length	0.029
RMSD bond angle	2.600
Data reduction software	HKL-2000
Data scaling software	SCALEPACK (2.2.0)
Phasing software	MOLREP (11.4.05; 02.04.2016)
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.620
High resolution limit [Å]	1.560	3.360	1.560
Rmerge	0.035	0.023	0.337
Rmeas	0.039	0.026	0.381
Rpim	0.016	0.011	0.170
Number of reflections	198652	19170	19986
<I/σ(I)>	24		4.37
Completeness [%]	98.6	92.2	100
Redundancy	4.7	5.2	4.5
CC(1/2)	0.999	0.999	0.906

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	2.0M Sodium malonate