5YP4
Crystal structure of dipeptidyl peptidase IV (DPP IV) with Lys-Pro from Pseudoxanthomonas mexicana WO24
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2013-11-20 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 |
| Unit cell lengths | 88.656, 104.487, 112.838 |
| Unit cell angles | 67.42, 68.83, 65.46 |
Refinement procedure
| Resolution | 40.000 - 1.900 |
| R-factor | 0.16635 |
| Rwork | 0.164 |
| R-free | 0.21320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5yp1 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.887 |
| Data reduction software | xia2 (0.5.436) |
| Data scaling software | SCALA (3.3.22) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.082 | 0.316 |
| Rmeas | 0.110 | 0.425 |
| Rpim | 0.073 | 0.282 |
| Number of reflections | 245086 | 27199 |
| <I/σ(I)> | 6.5 | 2 |
| Completeness [%] | 94.2 | 71.5 |
| Redundancy | 2.2 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293.15 | 12% PEG 20000, 80mM MES pH6.5, 20% Glycerol, 4mM Lys-Pro |
