5YLB

Crystal structure of Ofd2 from Schizosaccharomyces pombe at 1.80 A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-11-17
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97907
Spacegroup name	I 2 2 2
Unit cell lengths	70.506, 80.386, 81.996
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	57.400 - 1.790
R-factor	0.1876
Rwork	0.185
R-free	0.23290
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3thp
RMSD bond length	0.012
RMSD bond angle	1.180
Data reduction software	DENZO
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall
Low resolution limit [Å]	57.400
High resolution limit [Å]	1.790
Rmerge	0.079
Number of reflections	20695
<I/σ(I)>	2.69
Completeness [%]	99.9
Redundancy	8.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	289	18% MPEG 5000, 0.1M HEPES,pH7.0