5YKH
Crystal structure of the engineered nine-repeat PUF domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-05 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97915 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 203.052, 32.935, 61.364 |
| Unit cell angles | 90.00, 105.31, 90.00 |
Refinement procedure
| Resolution | 33.617 - 2.457 |
| R-factor | 0.2155 |
| Rwork | 0.213 |
| R-free | 0.25840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1m8z |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.435 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.106 | 0.461 |
| Rmeas | 0.115 | 0.495 |
| Rpim | 0.045 | 0.181 |
| Number of reflections | 14533 | 745 |
| <I/σ(I)> | 14.9 | 8.2 |
| Completeness [%] | 98.5 | 100 |
| Redundancy | 6.9 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 20% PEG 3350, 0.25M NaH2PO4 |
