5YJM
Human chymase in complex with 7-oxo-3-(phenoxyimino)-1,4-diazepane derivative
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-002 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2007-05-18 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.54178 |
Spacegroup name | P 43 |
Unit cell lengths | 75.089, 75.089, 49.750 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.970 - 1.900 |
R-factor | 0.14899 |
Rwork | 0.147 |
R-free | 0.18562 |
RMSD bond length | 0.026 |
RMSD bond angle | 2.494 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AMoRE |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 100.000 |
High resolution limit [Å] | 1.900 |
Number of reflections | 21174 |
<I/σ(I)> | 17.6 |
Completeness [%] | 95.9 |
Redundancy | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 293 | 22% PEG8000, 5mM zinc chloride, 100mM MES buffer |