5YJJ
Crystal structure of PNPase from Staphylococcus epidermidis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-11-25 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.95372 |
Spacegroup name | P 1 |
Unit cell lengths | 93.190, 93.160, 143.150 |
Unit cell angles | 73.54, 87.61, 60.07 |
Refinement procedure
Resolution | 73.040 - 2.200 |
R-factor | 0.3 |
Rwork | 0.237 |
R-free | 0.25900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3cdi |
RMSD bond length | 0.006 |
RMSD bond angle | 0.855 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 75.030 |
High resolution limit [Å] | 2.200 |
Number of reflections | 196762 |
<I/σ(I)> | 8.5 |
Completeness [%] | 97.6 |
Redundancy | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 7.3 | 295 | 0.2M Potassium formate, 20% PEG 3350, pH 7.3 |