5YJH
Structural insights into periostin functions
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-07-11 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.987 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 105.529, 105.529, 330.342 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 44.041 - 2.957 |
R-factor | 0.2148 |
Rwork | 0.213 |
R-free | 0.24180 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ltb |
RMSD bond length | 0.004 |
RMSD bond angle | 0.610 |
Data scaling software | HKL-3000 |
Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.010 |
High resolution limit [Å] | 2.957 | 2.957 |
Rmerge | 0.114 | 0.547 |
Rmeas | 0.131 | 0.626 |
Rpim | 0.062 | 0.295 |
Number of reflections | 22983 | 1117 |
<I/σ(I)> | 14.1 | 2.8 |
Completeness [%] | 96.4 | 96.5 |
Redundancy | 3.9 | 4 |
CC(1/2) | 0.876 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | Ammonium Sulfate. Sodium cacodylate |