5YJ2

Crystal structure of Bacillus sp. TB-90 urate oxidase without dehydration

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2012-11-14
Detector	ADSC QUANTUM 210
Wavelength(s)	1
Spacegroup name	P 21 21 2
Unit cell lengths	133.073, 143.895, 70.231
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.114 - 1.710
R-factor	0.1653
Rwork	0.163
R-free	0.19020
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.754
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.740
High resolution limit [Å]	1.710	1.710
Rmerge	0.102	0.760
Rmeas	0.107
Rpim	0.042	0.397
Number of reflections	147456	7291
<I/σ(I)>	15.8	2.38
Completeness [%]	99.8	99.9
Redundancy	6.7	6.3
CC(1/2)		0.809

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	288	16% PEG 8000, 0.1 M Tris-HCl, 0.08 M K2SO4, 2 mM 8-azaxanthine