5YIW
Caulobacter crescentus GcrA DNA-binding domain (DBD) in complex with methylated dsDNA (crystal form 2)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-05-08 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.979 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 49.810, 63.658, 126.730 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.745 - 1.551 |
R-factor | 0.2052 |
Rwork | 0.204 |
R-free | 0.23400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5yiu |
RMSD bond length | 0.006 |
RMSD bond angle | 1.008 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.580 |
High resolution limit [Å] | 1.550 | 1.550 |
Rmerge | 0.070 | 0.740 |
Rmeas | 0.070 | 0.800 |
Rpim | 0.030 | 0.300 |
Number of reflections | 29530 | 1394 |
<I/σ(I)> | 22.5 | 2.5 |
Completeness [%] | 99.6 | 99.9 |
Redundancy | 7.2 | 6.9 |
CC(1/2) | 0.880 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | 20% PEG 8000, 0.05M Potassium phosphate monobasic |