5YIW

Caulobacter crescentus GcrA DNA-binding domain (DBD) in complex with methylated dsDNA (crystal form 2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-05-08
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.979
Spacegroup name	C 2 2 21
Unit cell lengths	49.810, 63.658, 126.730
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.745 - 1.551
R-factor	0.2052
Rwork	0.204
R-free	0.23400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5yiu
RMSD bond length	0.006
RMSD bond angle	1.008
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.580
High resolution limit [Å]	1.550	1.550
Rmerge	0.070	0.740
Rmeas	0.070	0.800
Rpim	0.030	0.300
Number of reflections	29530	1394
<I/σ(I)>	22.5	2.5
Completeness [%]	99.6	99.9
Redundancy	7.2	6.9
CC(1/2)		0.880

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	20% PEG 8000, 0.05M Potassium phosphate monobasic