5YH3
The structure of hFam20C and hFam20A complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-10-02 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.977 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 78.469, 192.747, 226.669 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.557 - 3.300 |
R-factor | 0.2343 |
Rwork | 0.233 |
R-free | 0.26580 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 0.719 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 3.300 |
Number of reflections | 75772 |
<I/σ(I)> | 10 |
Completeness [%] | 99.7 |
Redundancy | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 290 | 0.1M BICINE pH 9.0, 2%(v/v) 1,4-Dioxane, 7%(w/v) Polyethylene glycol 20,000 |