5YH2
The structure of DrFam20C1 and hFam20A complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-10-02 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.977 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 110.059, 110.059, 461.698 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.943 - 3.550 |
R-factor | 0.248 |
Rwork | 0.245 |
R-free | 0.29840 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.739 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 3.550 |
Number of reflections | 35615 |
<I/σ(I)> | 11 |
Completeness [%] | 100.0 |
Redundancy | 11.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 290 | 11% v/v 2-Propanol, 0.1 M Sodium citrate tribasic dihydrate pH 5.0, 8% w/v Polyethylene glycol 10,000 |