5YGV
Crystal structure of the abscisic acid receptor PYR1 in complex with an antagonist
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-03-09 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 41 |
Unit cell lengths | 38.203, 38.203, 263.349 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.000 - 2.500 |
R-factor | 0.25758 |
Rwork | 0.256 |
R-free | 0.29395 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3wg8 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.326 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.540 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.114 | 0.536 |
Number of reflections | 12089 | 638 |
<I/σ(I)> | 20.7 | |
Completeness [%] | 98.9 | 93.7 |
Redundancy | 3.3 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1 M MES, pH 6.5, 16% PEG 3000 |