5YFV
Crystal structure of ribose-1,5-bisphosphate isomerase mutant C135S from Pyrococcus horikoshii OT3 in complex with ribose-1,5-bisphosphate and AMP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2016-12-03 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 31 1 2 |
Unit cell lengths | 98.140, 98.140, 255.681 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 85.230 - 2.750 |
R-factor | 0.1555 |
Rwork | 0.152 |
R-free | 0.22400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5yfj |
RMSD bond length | 0.013 |
RMSD bond angle | 1.744 |
Data scaling software | Aimless (0.5.28) |
Phasing software | PHASER (2.6.1) |
Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 85.230 | 2.870 | |
High resolution limit [Å] | 2.750 | 2.750 | |
Rmerge | 0.154 | 0.541 | 0.059 |
Rmeas | 0.165 | 0.578 | 0.064 |
Rpim | 0.058 | 0.204 | 0.024 |
Number of reflections | 37157 | 4510 | 979 |
<I/σ(I)> | 12 | 3.9 | |
Completeness [%] | 100.0 | 100 | 100 |
Redundancy | 7.9 | 7.9 | 7 |
CC(1/2) | 0.995 | 0.910 | 0.998 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.7 M NaCl, 3% PEG 6000, 25% MPD, 0.1% Low Melting Agarose |