5YFJ

Crystal structure of ribose-1,5-bisphosphate isomerase from Pyrococcus horikoshii OT3 in complex with ribulose-1,5-bisphosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2016-10-31
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 31 1 2
Unit cell lengths	98.630, 98.630, 257.061
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	85.690 - 2.200
R-factor	0.1517
Rwork	0.150
R-free	0.18970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3vm6
RMSD bond length	0.018
RMSD bond angle	1.903
Data scaling software	Aimless (7.2.1)
Phasing software	PHASER (2.6.1)
Refinement software	REFMAC (5.8.0155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	85.690	2.250
High resolution limit [Å]	2.200	2.200
Rmerge	0.092	0.536	0.034
Rmeas	0.098	0.566	0.036
Rpim	0.031	0.180	0.012
Number of reflections	72651	4463	692
<I/σ(I)>	17.8	4.5
Completeness [%]	99.5	98.7	99.7
Redundancy	9.9	9.7	8.9
CC(1/2)	0.998	0.923	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.7 M NaCl, 3% PEG 6000, 25% MPD