5YFJ
Crystal structure of ribose-1,5-bisphosphate isomerase from Pyrococcus horikoshii OT3 in complex with ribulose-1,5-bisphosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2016-10-31 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 31 1 2 |
Unit cell lengths | 98.630, 98.630, 257.061 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 85.690 - 2.200 |
R-factor | 0.1517 |
Rwork | 0.150 |
R-free | 0.18970 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3vm6 |
RMSD bond length | 0.018 |
RMSD bond angle | 1.903 |
Data scaling software | Aimless (7.2.1) |
Phasing software | PHASER (2.6.1) |
Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 85.690 | 2.250 | |
High resolution limit [Å] | 2.200 | 2.200 | |
Rmerge | 0.092 | 0.536 | 0.034 |
Rmeas | 0.098 | 0.566 | 0.036 |
Rpim | 0.031 | 0.180 | 0.012 |
Number of reflections | 72651 | 4463 | 692 |
<I/σ(I)> | 17.8 | 4.5 | |
Completeness [%] | 99.5 | 98.7 | 99.7 |
Redundancy | 9.9 | 9.7 | 8.9 |
CC(1/2) | 0.998 | 0.923 | 0.998 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.7 M NaCl, 3% PEG 6000, 25% MPD |