5YF9
Crystal structure of CK2a2 form-2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 95 |
| Detector technology | PIXEL |
| Collection date | 2016-06-19 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.468, 102.422, 72.229 |
| Unit cell angles | 90.00, 95.02, 90.00 |
Refinement procedure
| Resolution | 46.289 - 1.890 |
| R-factor | 0.2142 |
| Rwork | 0.211 |
| R-free | 0.26310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ofm |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.058 |
| Data reduction software | HKL-2000 (9) |
| Data scaling software | HKL-2000 (9) |
| Phasing software | MOLREP (11.2.05) |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 46.289 |
| High resolution limit [Å] | 1.890 |
| Number of reflections | 53854 |
| <I/σ(I)> | 10 |
| Completeness [%] | 99.9 |
| Redundancy | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | 24%w/v PEG 4000, 0.15 M Lithium Sulfate mono hydrate, 0.1 M Tris-HCl (pH 8.5) |
