5YEI
Mechanistic insight into the regulation of Pseudomonas aeruginosa aspartate kinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-04-11 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97776 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 82.459, 171.793, 88.932 |
| Unit cell angles | 90.00, 90.29, 90.00 |
Refinement procedure
| Resolution | 40.091 - 2.301 |
| R-factor | 0.2239 |
| Rwork | 0.222 |
| R-free | 0.25740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3aaw |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.715 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1-2155_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.900 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 106886 | |
| <I/σ(I)> | 7.52 | 1.94 |
| Completeness [%] | 97.7 | 91.8 |
| Redundancy | 3.8 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 292 | 0.4 M ammonium sulfate 0.1 M sodium citrate tribasic dihydrate pH 5.6 0.9 M lithium sulfate monohydrate |
