5YEH
Crystal structure of CTCF ZFs4-8-eCBS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-11-07 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9785 |
| Spacegroup name | P 1 |
| Unit cell lengths | 45.845, 54.876, 66.938 |
| Unit cell angles | 81.17, 80.11, 79.72 |
Refinement procedure
| Resolution | 32.706 - 2.328 |
| R-factor | 0.2243 |
| Rwork | 0.222 |
| R-free | 0.26950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5yeg |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.208 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.710 | 2.380 |
| High resolution limit [Å] | 2.330 | 2.340 |
| Rmerge | 0.073 | 0.660 |
| Rpim | 0.034 | 0.306 |
| Number of reflections | 25836 | |
| <I/σ(I)> | 20.2 | 2.54 |
| Completeness [%] | 96.5 | 97.4 |
| Redundancy | 5.4 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 289 | 0.1 M Bis-tris pH 5.6-6.0, 0.2 M Sodium chloride and 17-24% PEG 3350 |
