5YDK
Crystal structure of RNF168 UDM1 in complex with Lys63-linked diubiquitin, tetrameric form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-04-18 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 85.344, 64.120, 117.464 |
| Unit cell angles | 90.00, 109.62, 90.00 |
Refinement procedure
| Resolution | 42.654 - 2.505 |
| R-factor | 0.231 |
| Rwork | 0.230 |
| R-free | 0.25950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fid |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.587 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 40288 | |
| <I/σ(I)> | 6.875 | 1.25 |
| Completeness [%] | 97.2 | 97.4 |
| Redundancy | 5.1 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M Tris pH 7.6 21% PEG3350 |
