5YD3
Crystal structure of the scFv antibody 4B08 with epitope peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-11-04 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 80.480, 72.970, 86.990 |
Unit cell angles | 90.00, 114.30, 90.00 |
Refinement procedure
Resolution | 33.100 - 1.350 |
R-factor | 0.13633 |
Rwork | 0.136 |
R-free | 0.18397 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1qok |
RMSD bond length | 0.014 |
RMSD bond angle | 1.758 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.100 | 1.420 |
High resolution limit [Å] | 1.350 | 1.350 |
Rmerge | 0.083 | 0.519 |
Rpim | 0.050 | 0.308 |
Number of reflections | 193517 | 27587 |
<I/σ(I)> | 9.7 | 3.8 |
Completeness [%] | 96.5 | 94.4 |
Redundancy | 3.7 | 3.8 |
CC(1/2) | 0.995 | 0.759 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293.15 | 100 mM BIS-TRIS, 1.8 M Ammonium sulphate |