5Y9M
Crystal structure of CK2a2 form 3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL32XU |
| Synchrotron site | SPring-8 |
| Beamline | BL32XU |
| Temperature [K] | 95 |
| Detector technology | PIXEL |
| Collection date | 2016-10-21 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 72.766, 92.052, 102.891 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.917 - 2.006 |
| R-factor | 0.1729 |
| Rwork | 0.171 |
| R-free | 0.20470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3e3b |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.099 |
| Data reduction software | XDS (Nov 1, 2016) |
| Data scaling software | XDS (Nov 1, 2016) |
| Phasing software | PHASER (2.5.6) |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.917 | 2.130 |
| High resolution limit [Å] | 2.006 | 2.010 |
| Rmerge | 0.420 | |
| Number of reflections | 89053 | |
| <I/σ(I)> | 7 | 7.01 |
| Completeness [%] | 99.6 | 98.8 |
| Redundancy | 3.5 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 281 | 26% PEG 4000, 0.15 M Lithium sulfate monohydrate, 0.1 M TRIS hydrochloride (pH 8.5) |
