5Y9K

Structure of the belimumab Fab fragment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2016-09-10
Detector	ADSC QUANTUM 270
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	60.912, 52.279, 63.748
Unit cell angles	90.00, 90.72, 90.00

Refinement procedure

Resolution	26.314 - 1.900
R-factor	0.1686
Rwork	0.167
R-free	0.20570
RMSD bond length	0.007
RMSD bond angle	1.096
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.900
Number of reflections	31056
<I/σ(I)>	40.2
Completeness [%]	97.7
Redundancy	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	293	0.1 M HEPES pH 7.5, 0.2 M lithium sulfate monohydrate, 25% (w/v)polyethylene glycol 3,350