5Y9J

BAFF in complex with belimumab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2016-09-14
Detector	ADSC QUANTUM 315r
Wavelength(s)	1
Spacegroup name	P 21 3
Unit cell lengths	134.001, 134.001, 134.001
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.280 - 2.050
R-factor	0.1816
Rwork	0.180
R-free	0.21680
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.129
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.050
Number of reflections	48698
<I/σ(I)>	52.3
Completeness [%]	97.7
Redundancy	14.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8.5	293	0.1 M Tris(hydroxymethyl)aminomethane pH 8.5, 20% (w/v)polyethylene glycol 1,000