5Y90
MAP2K7 mutant -C218S
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-06-19 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 52.396, 70.607, 92.155 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 56.050 - 1.300 |
| R-factor | 0.12887 |
| Rwork | 0.127 |
| R-free | 0.16714 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5b2l |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.141 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.050 | 1.320 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.028 | 0.878 |
| Number of reflections | 84643 | |
| <I/σ(I)> | 60.4 | 1.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.4 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | PEG3350, sodium citrate |
