5Y8Z
Crystal Structure Analysis of the BRD4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2015-09-07 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97853 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 39.480, 48.270, 77.890 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.030 - 1.840 |
R-factor | 0.1687 |
Rwork | 0.168 |
R-free | 0.18450 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.510 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless (0.5.14) |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.270 | 1.880 |
High resolution limit [Å] | 1.840 | 1.840 |
Rmerge | 0.125 | 0.273 |
Rmeas | 0.131 | 0.285 |
Rpim | 0.037 | 0.081 |
Number of reflections | 13419 | 835 |
<I/σ(I)> | 16 | |
Completeness [%] | 99.4 | 99.2 |
Redundancy | 11.7 | 12 |
CC(1/2) | 0.993 | 0.989 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 277 | 20% PEG3350, 0.2M NaNO3, 0.1M HEPES, 10% EtGhly,pH7.2 |