5Y86
Crystal structure of kinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 77.15 |
| Detector technology | CCD |
| Collection date | 2015-04-09 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.97933 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 43.000, 76.520, 148.470 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.900 |
| R-factor | 0.16833 |
| Rwork | 0.167 |
| R-free | 0.19642 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3k2l |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.167 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 1.850 |
| Number of reflections | 628287 |
| <I/σ(I)> | 11 |
| Completeness [%] | 99.6 |
| Redundancy | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.2 | 290.15 | 1M NaH2PO4 and K2HPO4 (pH8.2) |
