5Y85
Structure-based Insights into Self-Cleavage by a Four-way Junctional Twister-Sister Ribozyme
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-01-01 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.987 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 91.182, 109.304, 41.739 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.852 - 2.001 |
R-factor | 0.2189 |
Rwork | 0.218 |
R-free | 0.23740 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.018 |
RMSD bond angle | 0.400 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.000 |
Number of reflections | 28779 |
<I/σ(I)> | 16.4 |
Completeness [%] | 99.7 |
Redundancy | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M NaOAc, pH 5.2, 0.15M CaCl2, 18-22% iso-propanol or MPD |