5Y7A
Crystal structure of Pseudomonas fluorescens Kynurenine 3-monooxygenase in complex with L-KYN
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-06-16 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.9791 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 68.480, 46.513, 71.311 |
Unit cell angles | 90.00, 104.34, 90.00 |
Refinement procedure
Resolution | 38.584 - 1.846 |
R-factor | 0.1759 |
Rwork | 0.174 |
R-free | 0.21740 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5y77 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.335 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.846 |
Number of reflections | 36572 |
<I/σ(I)> | 33.2 |
Completeness [%] | 97.6 |
Redundancy | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 6.5 | 293 | 0.1 M Bis-Tris at pH 6.5, 18% w/v PEG3350, and 0.2 M NaCl |