5Y77
Crystal structure of Pseudomonas fluorescens Kynurenine 3-monooxygenase in complex with L-KYN (seMet derivative)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-02-11 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9789 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.555, 52.448, 135.712 |
| Unit cell angles | 90.00, 104.33, 90.00 |
Refinement procedure
| Resolution | 34.776 - 1.600 |
| R-factor | 0.1867 |
| Rwork | 0.185 |
| R-free | 0.21720 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.956 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.600 |
| Number of reflections | 232059 |
| <I/σ(I)> | 23.6 |
| Completeness [%] | 94.7 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 6 | 293 | 20% w/v PEG3350, 0.2 M NaCl, 0.1 M Bis-Tris at pH 6.0 and 5 mM DTT |
