5Y5W
Crystal structure of human Spindlin1 in complex with a histone H4K20(me3) peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-05-21 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9791 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 88.769, 148.504, 169.212 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 84.610 - 3.300 |
| R-factor | 0.22387 |
| Rwork | 0.219 |
| R-free | 0.30810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ns2 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.782 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 84.610 | 3.360 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.130 | 0.450 |
| Number of reflections | 17175 | 822 |
| <I/σ(I)> | 29.2 | 10.2 |
| Completeness [%] | 99.0 | 100 |
| Redundancy | 9.9 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 1.5 M (NH4)2SO4, 0.1 M Bis-Tris |
