5Y58
Crystal structure of Ku70/80 and TLC1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-05-23 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97853 |
| Spacegroup name | P 1 |
| Unit cell lengths | 116.296, 115.159, 115.847 |
| Unit cell angles | 77.43, 78.48, 63.73 |
Refinement procedure
| Resolution | 44.107 - 2.800 |
| R-factor | 0.2175 |
| Rwork | 0.215 |
| R-free | 0.25770 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.587 |
| Data scaling software | HKL-3000 |
| Phasing software | XDS |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 6.030 | 2.800 |
| Rmerge | 0.076 | 0.039 | 0.556 |
| Rmeas | 0.089 | 0.046 | 0.645 |
| Rpim | 0.045 | 0.023 | 0.326 |
| Total number of observations | 463727 | ||
| Number of reflections | 121941 | 12863 | 12530 |
| <I/σ(I)> | 6.6 | ||
| Completeness [%] | 93.9 | 99.1 | 96 |
| Redundancy | 3.8 | 3.9 | 3.8 |
| CC(1/2) | 0.996 | 0.803 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 8 | 277 | 0.2 M calcium acetate, 0.1 M imidazole, 10% PEG8000 |
| 1 | EVAPORATION | 8 | 277 | 0.2 M calcium acetate, 0.1 M imidazole, 10% PEG8000 |
