5Y4Z
Crystal structure of the Zika virus NS3 helicase complex with AMPPNP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-05-06 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9785 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 53.754, 68.779, 56.971 |
Unit cell angles | 90.00, 92.50, 90.00 |
Refinement procedure
Resolution | 39.939 - 1.300 |
R-factor | 0.1928 |
Rwork | 0.192 |
R-free | 0.21580 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.198 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.300 |
Number of reflections | 75138 |
<I/σ(I)> | 26.1 |
Completeness [%] | 73.5 |
Redundancy | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 8% Tacsimate 6.0, 20% PEG 3350 |