5Y4Z
Crystal structure of the Zika virus NS3 helicase complex with AMPPNP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-05-06 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9785 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.754, 68.779, 56.971 |
| Unit cell angles | 90.00, 92.50, 90.00 |
Refinement procedure
| Resolution | 39.939 - 1.300 |
| R-factor | 0.1928 |
| Rwork | 0.192 |
| R-free | 0.21580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.198 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.300 |
| Number of reflections | 75138 |
| <I/σ(I)> | 26.1 |
| Completeness [%] | 73.5 |
| Redundancy | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 8% Tacsimate 6.0, 20% PEG 3350 |
