5Y4R

Structure of a methyltransferase complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-25
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.953726
Spacegroup name	P 21 21 21
Unit cell lengths	88.521, 98.927, 110.646
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.285 - 2.298
R-factor	0.1973
Rwork	0.193
R-free	0.23960
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ftw
RMSD bond length	0.005
RMSD bond angle	0.964
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.440
High resolution limit [Å]	2.298	2.300
Number of reflections	43900	6939
<I/σ(I)>	12.64	1.43
Completeness [%]	99.8	99
Redundancy	6.75	6.91
CC(1/2)	0.998	0.568

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	Bis-Tris, PEG 3350, lithium salfate