5Y3A

Crystal structure of Ragulator complex (p18 49-161)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-11-01
Detector	RAYONIX MX225HE
Wavelength(s)	0.9793
Spacegroup name	P 21 21 21
Unit cell lengths	71.341, 89.626, 232.173
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.010 - 2.900
R-factor	0.24788
Rwork	0.246
R-free	0.28849
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ms6 1vet
RMSD bond length	0.007
RMSD bond angle	1.336
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0123)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.010	50.010	3.000
High resolution limit [Å]	2.900	6.240	2.900
Rmerge	0.105	0.042	0.540
Rmeas	0.120	0.048	0.616
Rpim	0.056	0.022	0.291
Number of reflections	30426
<I/σ(I)>	10.1
Completeness [%]	90.0	97.7	88.4
Redundancy	4.1	4.4	4.2
CC(1/2)		0.998	0.841

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	289	0.1 M Bis-Tris (pH 5.5), and 25% (w/v) PEG 3350