5Y3A
Crystal structure of Ragulator complex (p18 49-161)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-11-01 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 0.9793 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 71.341, 89.626, 232.173 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.010 | 50.010 | 3.000 |
High resolution limit [Å] | 2.900 | 6.240 | 2.900 |
Rmerge | 0.105 | 0.042 | 0.540 |
Rmeas | 0.120 | 0.048 | 0.616 |
Rpim | 0.056 | 0.022 | 0.291 |
Number of reflections | 30426 | ||
<I/σ(I)> | 10.1 | ||
Completeness [%] | 90.0 | 97.7 | 88.4 |
Redundancy | 4.1 | 4.4 | 4.2 |
CC(1/2) | 0.998 | 0.841 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 289 | 0.1 M Bis-Tris (pH 5.5), and 25% (w/v) PEG 3350 |