5Y39

Crystal structure of Ragulator complex (p18 76-145)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-05-06
Detector	RAYONIX MX225HE
Wavelength(s)	0.9792
Spacegroup name	C 2 2 21
Unit cell lengths	110.659, 117.854, 157.805
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.010 - 2.650
R-factor	0.21799
Rwork	0.216
R-free	0.26260
RMSD bond length	0.006
RMSD bond angle	1.167
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0123)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.010	50.000	2.740
High resolution limit [Å]	2.650	5.710	2.650
Rmerge	0.046	0.022	0.509
Rmeas	0.052	0.025	0.576
Rpim	0.023	0.011	0.262
Number of reflections	29802	3028	2971
<I/σ(I)>	12.6
Completeness [%]	98.1	94.3	98.6
Redundancy	4.6	4.4	4.6
CC(1/2)		0.997	0.801

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	289	0.1 M Bis-Tris (pH 5.5), and 25% (w/v) PEG 3350