5Y37
Crystal structure of GBS GAPDH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 190 |
| Detector technology | CCD |
| Collection date | 2014-07-02 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 144.300, 156.740, 67.820 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.983 - 1.360 |
| R-factor | 0.1665 |
| Rwork | 0.166 |
| R-free | 0.18500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4qx6 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.904 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_2481) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.440 |
| High resolution limit [Å] | 1.360 | 1.360 |
| Number of reflections | 326358 | 50753 |
| <I/σ(I)> | 15.87 | |
| Completeness [%] | 99.3 | 96.2 |
| Redundancy | 6.83 | 6.1 |
| CC(1/2) | 0.999 | 0.683 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 2M Ammonium Sulfate, 0.1 M Tris-HCl, pH 8.5 |
