5Y2P
Crystal Structure of Bacillus sp. TB-90 Urate Oxidase Improved by Humidity Control at 89% RH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-05-22 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 71.095, 133.885, 145.797 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.121 - 1.500 |
| R-factor | 0.1584 |
| Rwork | 0.157 |
| R-free | 0.17690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3wlv |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.091 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.12_2829)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.121 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.077 | 0.895 |
| Rpim | 0.031 | 0.367 |
| Number of reflections | 111066 | 5500 |
| <I/σ(I)> | 34.5 | 2.47 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.2 | 6.9 |
| CC(1/2) | 0.780 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 0.1 M Tris-HCl, 0.06 M K2SO4, 16% (w/v) PEG 8000 |
