5Y20
Crystal structure of AL1 PHD finger bound to H3K4me3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-03-18 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9792 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 46.168, 46.168, 70.164 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.568 - 2.409 |
R-factor | 0.1685 |
Rwork | 0.161 |
R-free | 0.21510 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2fsa |
RMSD bond length | 0.007 |
RMSD bond angle | 1.002 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (phenix.refine: 1.9_1692) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.440 |
High resolution limit [Å] | 2.400 | 6.510 | 2.400 |
Rmerge | 0.221 | 0.087 | 0.844 |
Rmeas | 0.225 | 0.089 | 0.859 |
Rpim | 0.043 | 0.018 | 0.162 |
Number of reflections | 3265 | ||
<I/σ(I)> | 3.8 | ||
Completeness [%] | 100.0 | 100 | 100 |
Redundancy | 26.5 | 21.4 | 27.7 |
CC(1/2) | 0.999 | 0.987 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | 0.1M Tris pH 8., 3.0M NaCl |