5Y0C

Crystal Structure of the human nucleosome at 2.09 angstrom resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-06-25
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.1000
Spacegroup name	P 21 21 21
Unit cell lengths	98.913, 107.103, 167.003
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.087
R-factor	0.2028
Rwork	0.202
R-free	0.24920
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2cv5
Data scaling software	HKL-2000
Phasing software	PHASER (2.5.5)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	20.000	20.000	2.150
High resolution limit [Å]	2.080	4.470	2.080
Rmerge	0.100	0.086	0.487
Rmeas	0.105	0.089	0.532
Rpim	0.032	0.024	0.207
Number of reflections	103710
<I/σ(I)>	10.3
Completeness [%]	98.5	100	91.2
Redundancy	8.7	13	5.4
CC(1/2)		0.997	0.685

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	potassium cacodylate, potassium chloride, manganese chloride