5XX7
Hetero-micro-seeding: Crystal structure of BPTI-[5,55]C14GA38I variant using micro-seeds from -C14GA38I variant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
Synchrotron site | Photon Factory |
Beamline | AR-NE3A |
Temperature [K] | 101 |
Detector technology | CCD |
Collection date | 2010-11-26 |
Detector | AGILENT EOS CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 25.189, 41.306, 53.940 |
Unit cell angles | 90.00, 99.35, 90.00 |
Refinement procedure
Resolution | 53.220 - 1.380 |
R-factor | 0.17828 |
Rwork | 0.176 |
R-free | 0.21842 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2zvx |
RMSD bond length | 0.026 |
RMSD bond angle | 2.474 |
Data reduction software | DENZO |
Data scaling software | SCALA |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 53.220 | 1.430 |
High resolution limit [Å] | 1.380 | 1.380 |
Rmerge | 0.279 | |
Number of reflections | 22666 | |
<I/σ(I)> | 4.41 | 4.41 |
Completeness [%] | 98.1 | 98.1 |
Redundancy | 3.6 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 20-30% PEG4000; 0.2M Lithium Sulfate; 0.1M Tris-HCL, H8.5 |