5XX2
A BPTI-[5,55] variant with C14GA38L mutations
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 101 |
| Detector technology | CCD |
| Collection date | 2010-10-25 |
| Detector | AGILENT EOS CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 25.413, 40.690, 61.147 |
| Unit cell angles | 90.00, 89.99, 90.00 |
Refinement procedure
| Resolution | 61.150 - 1.120 |
| R-factor | 0.13126 |
| Rwork | 0.130 |
| R-free | 0.14575 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zvx |
| RMSD bond length | 0.038 |
| RMSD bond angle | 2.988 |
| Data reduction software | DENZO |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.150 | 1.040 |
| High resolution limit [Å] | 1.000 | 1.000 |
| Rmerge | 0.289 | |
| Number of reflections | 67481 | |
| <I/σ(I)> | 6.32 | 6.32 |
| Completeness [%] | 99.5 | 99.9 |
| Redundancy | 6.4 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293.15 | 30% PEG4000; 0.2M Lithium Sulfate; 0.1M Tris-HCl, pH8.5 |
