5XVV
Crystal Structure of Forward Inhibited Aspergillus niger Glutamate Dehydrogenase With Both Apo- and Alpha Ketoglutarate Bound Subunits
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2015-10-08 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 |
Unit cell lengths | 92.260, 92.150, 111.060 |
Unit cell angles | 103.40, 93.95, 120.36 |
Refinement procedure
Resolution | 33.000 - 2.250 |
R-factor | 0.16029 |
Rwork | 0.158 |
R-free | 0.19863 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 1.433 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.000 | 2.350 |
High resolution limit [Å] | 2.250 | 2.250 |
Rmerge | 0.095 | 0.390 |
Number of reflections | 135737 | 15991 |
<I/σ(I)> | 7.96 | 2.1 |
Completeness [%] | 95.5 | 92 |
Redundancy | 2 | |
CC(1/2) | 0.990 | 0.680 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.15 M potassium bromide, 20% w/v PEG 2000 MME |