5XVS
Crystal structure of UDP-GlcNAc 2-epimerase NeuC complexed with UDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-07-12 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 86.879, 147.654, 124.981 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.960 - 2.383 |
| R-factor | 0.1733 |
| Rwork | 0.171 |
| R-free | 0.21080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4zht |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.764 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 27.950 |
| High resolution limit [Å] | 2.380 |
| Number of reflections | 32255 |
| <I/σ(I)> | 9.22 |
| Completeness [%] | 99.5 |
| Redundancy | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 0.2 M Li2SO4, 0.1 M Tris-HCl pH 8.5, 25% PEG 3350 |
