5XUS

Crystal structure of Lachnospiraceae bacterium ND2006 Cpf1 in complex with crRNA and target DNA (TTTA PAM)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-08-29
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00
Spacegroup name	P 41 21 2
Unit cell lengths	103.205, 103.205, 363.909
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.640 - 2.500
R-factor	0.1807
Rwork	0.178
R-free	0.22760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5id6
RMSD bond length	0.007
RMSD bond angle	0.913
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX ((1.11.1_2575))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.640	2.560
High resolution limit [Å]	2.500	2.500
Number of reflections	69225
<I/σ(I)>	12.7
Completeness [%]	99.9
Redundancy	12.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	PEG3350, MIB buffer, pH 5.0