5XSK
Crystal structure of PWWP-DNA complex for human hepatoma-derived growth factor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE TPS 05A |
| Synchrotron site | NSRRC |
| Beamline | TPS 05A |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2016-11-06 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1 |
| Spacegroup name | P 31 |
| Unit cell lengths | 32.402, 32.402, 205.820 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.840 |
| R-factor | 0.19519 |
| Rwork | 0.191 |
| R-free | 0.26922 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5xsl |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.493 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.940 |
| High resolution limit [Å] | 2.840 | 2.840 |
| Rmerge | 0.109 | 0.585 |
| Number of reflections | 5853 | |
| <I/σ(I)> | 17.25 | 2.57 |
| Completeness [%] | 98.7 | 90.6 |
| Redundancy | 8.3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | Sodium nitrate, Sodium phosphate dibasic, Ammonium sulfate, Tris, Bicine, MPD, PEG 1000, PEG 3350 |
