5XRZ

Structure of a ssDNA bound to the inner DNA binding site of RAD52

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-12-07
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.10000
Spacegroup name	P 1
Unit cell lengths	67.621, 101.420, 101.710
Unit cell angles	91.89, 108.68, 108.17

Refinement procedure

Resolution	45.703 - 3.600
R-factor	0.2188
Rwork	0.216
R-free	0.25650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1kn0
RMSD bond length	0.002
RMSD bond angle	0.480
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	95.300	3.700
High resolution limit [Å]	3.600	3.600
Rmerge	0.103	0.400
Rmeas	0.145	0.565
Rpim	0.103	0.400
Number of reflections	27023	4412
<I/σ(I)>	7.5	2.7
Completeness [%]	96.9	97.1
Redundancy	3.3	3.4
CC(1/2)	0.972	0.597

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	calcium acetate, PEG 3,350