5XOX
Crystal structure of tRNA(His) guanylyltranserase from Saccharomyces cerevisiae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-12-04 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.97952 |
Spacegroup name | I 41 |
Unit cell lengths | 164.703, 164.703, 275.891 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.841 - 3.000 |
R-factor | 0.2403 |
Rwork | 0.239 |
R-free | 0.27370 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3otb |
RMSD bond length | 0.014 |
RMSD bond angle | 1.725 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.841 | 3.107 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.142 | 0.377 |
Number of reflections | 68293 | 12656 |
<I/σ(I)> | 17.87 | 3.02 |
Completeness [%] | 93.4 | 84 |
Redundancy | 5.1 | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.1M MES pH 6.0, 0.9M Ammonium sulfate |