5XOJ

Crystal structure of Xpo1p-PKI-Nup42p-Gsp1p-GTP complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2012-12-04
Detector	RAYONIX MX225HE
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	101.640, 107.560, 149.110
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.582 - 2.200
R-factor	0.1924
Rwork	0.191
R-free	0.21950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3wyg
RMSD bond length	0.002
RMSD bond angle	0.656
Data reduction software	iMOSFLM
Data scaling software	Aimless (0.1.27)
Phasing software	MOLREP
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	31.582	31.580	2.240
High resolution limit [Å]	2.200	11.430	2.200
Rmerge	0.119	0.032	0.798
Rpim	0.057	0.016	0.403
Number of reflections	81567
<I/σ(I)>	9.4	25.4	2
Completeness [%]	97.8	93.7	96.4
Redundancy	5.2	4.7	4.7
CC(1/2)	0.996	0.998	0.560

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.7	293	PEG20000, Tris-HCl