5XN6
Heterodimer crystal structure of geranylgeranyl diphosphate synthases 1 with GGPPS Recruiting Protein(OsGRP) from Oryza sativa
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17B1 |
Synchrotron site | SSRF |
Beamline | BL17B1 |
Temperature [K] | 77 |
Detector technology | PIXEL |
Collection date | 2013-09-10 |
Detector | CS-PAD CXI-1 |
Wavelength(s) | 0.9798 |
Spacegroup name | P 1 |
Unit cell lengths | 103.062, 102.981, 103.486 |
Unit cell angles | 109.39, 109.59, 109.13 |
Refinement procedure
Resolution | 37.755 - 3.598 |
R-factor | 0.2525 |
Rwork | 0.252 |
R-free | 0.26330 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5e8h |
RMSD bond length | 0.013 |
RMSD bond angle | 2.542 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 37.800 |
High resolution limit [Å] | 3.590 |
Number of reflections | 36920 |
<I/σ(I)> | 8.26 |
Completeness [%] | 96.9 |
Redundancy | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 16% PEG 10000, 0.1 M Bis-Tris, pH 6.5 |