5XN5
Homo-dimer crystal structure of geranylgeranyl diphosphate synthases 1 from Oryza sativa
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17B1 |
Synchrotron site | SSRF |
Beamline | BL17B1 |
Temperature [K] | 77 |
Detector technology | PIXEL |
Collection date | 2013-09-10 |
Detector | CS-PAD CXI-1 |
Wavelength(s) | 0.9798 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 56.022, 74.118, 71.288 |
Unit cell angles | 90.00, 90.81, 90.00 |
Refinement procedure
Resolution | 38.056 - 2.002 |
R-factor | 0.1884 |
Rwork | 0.187 |
R-free | 0.21350 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5e8h |
RMSD bond length | 0.011 |
RMSD bond angle | 1.374 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 38.100 |
High resolution limit [Å] | 2.000 |
Number of reflections | 39062 |
<I/σ(I)> | 19 |
Completeness [%] | 99.2 |
Redundancy | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293.15 | 2.0 M sodium malonate, 20% PEG 3350, pH 7.0 |